Team:TU Darmstadt/Notebook/sec1/K1602025
K1602025 - B0034-HistidinTag-Green fluorescent protein-SilicaTag4 (B0034-His-GFP-Si4)
K1602024 was digested with XbaI and PstI and ligated into B0034-pSB1A2 and transformed into E. coli Top 10. Colonies were screened by colony PCR with oligonucleotides VF2 and VR. Positive colonies were inoculated and the plasmids were extracted (Figure 1).
![](https://static.igem.org/mediawiki/2015/a/ac/Figure_3-_Colony_PCR_of_B0034-Prefix-His-Tag-GFP-Si4.png)
Figure 1 Colony PCR of B0034-Prefix-His-Tag-GFP-Si4 (1). Colony 1 contained the insert of around 1.1 kbp. DNA marker: 2-Log DNA Ladder.